Three types of routes are offered by Amtrak: long-distance routes, state-supported routes, and Northeast Corridor routes. The Northeast Corridor service is subsidized by federal appropriations, while federally-supported long-distance services are subsidized by appropriations under a separate line item from the NEC in federal budgets. Amtrak partners with 17 states to provide additional short- and medium-distance services, which are subsidized by periodic payments from state partners.
Three routes – Carolinian, Northeast Regional, and Vermonter – are state-subsidized only on sections of the Northeast Corridor. The Northeast Regional and San Joaquin have branches served by different trips, while the Empire Builder and Lake Shore Limited are split into two sections. Not every trip runs the full route.
| No | Name | Type | Route | Daily round trips | Route miles |
| 1 | Acela | NEC | Boston – New York – Washington | 16 (weekday), 4 (Sat), 9 (Sun) | 456 |
| 2 | Adirondack | State-supported | Montreal – New York | 1 | 381 |
| 3 | Amtrak Cascades | State-supported | Vancouver – Seattle – Portland – Eugene | 4 | 467 |
| 4 | Auto Train | Long distance | Lorton – Sanford | 1 | 855 |
| 5 | Berkshire Flyer | State-supported | New York – Pittsfield | 1 weekly round trip | 190 |
| 6 | Blue Water | State-supported | Chicago – Port Huron | 1 | 319 |
| 7 | California Zephyr | Long distance | Chicago – Emeryville, California | 1 | 2,438 |
| 8 | Capitol Corridor | State-supported | Auburn – Sacramento – Oakland – San Jose | 9 | 172 |
| 9 | Capitol Limited | Long distance | Chicago – Washington | 1 | 764 |
| 10 | Cardinal | Long distance | Chicago – New York | 3 weekly round trips | 1,147 |
| 11 | Carolinian | NEC; State-supported | New York – Charlotte | 1 | 704 |
| 12 | City of New Orleans | Long distance | Chicago – New Orleans | 1 | 926 |
| 13 | Coast Starlight | Long distance | Seattle – Los Angeles | 1 | 1,377 |
| 14 | Crescent | Long distance | New York City – New Orleans | 1 | 1,377 |
| 15 | Downeaster | State-supported | Brunswick – Portland – Boston | 5 | 145 |
| 16 | Empire Builder | Long distance | Chicago – Spokane – Portland/Seattle | 1 | 2,257 (Chicago–Portland)2,206 (Chicago–Seattle) |
| 17 | Empire Service | State-supported | New York – Albany – Niagara Falls | 7 (Sun-Fri), 6 (Sat) | 460 |
| 18 | Ethan Allen Express | State-supported | New York – Burlington | 1 | 308 |
| 19 | Hartford Line | State-supported | Springfield – New Haven | 6 (weekday), 4 (Sat), 5 (Sun) | 63 |
| 20 | Heartland Flyer | State-supported | Oklahoma City – Fort Worth | 1 | 206 |
| 21 | Hiawatha Service | State-supported | Chicago – Milwaukee | 7 (Monday-Saturday), 6 (Sunday) | 86 |
| 22 | Illini and Saluki | State-supported | Chicago – Carbondale | 2 | 310 |
| 23 | Illinois Zephyr and Carl Sandburg | State-supported | Chicago – Quincy | 2 | 258 |
| 24 | Keystone Service | State-supported | New York – Philadelphia – Harrisburg | 13 (weekday), 7 (weekend) | 195 |
| 25 | Lake Shore Limited | Long distance | New York/Boston – Albany – Chicago | 1 | 1,018 (Chicago – Boston)959 (Chicago – New York) |
| 26 | Lincoln Service | State-supported | Chicago – St. Louis | 4 | 284 |
| 27 | Maple Leaf | State-supported | New York – Toronto | 1 | 544 |
| 28 | Missouri River Runner | State-supported | St. Louis – Kansas City | 2 | 283 |
| 29 | Northeast Regional | NEC; State-supported | Boston/Springfield – New York – Washington –Norfolk/Newport News/Roanoke | 18 (weekday), 15 (weekend) | 644 (Boston – Newport News)679 (Boston – Norfolk)682 (Boston – Roanoke) |
| 30 | Pacific Surfliner | State-supported | San Luis Obispo – Goleta – Los Angeles – San Diego | 10 | 350 |
| 31 | Palmetto | Long distance | New York City – Savannah | 1 | 829 |
| 32 | Pennsylvanian | State-supported | New York – Pittsburgh | 1 | 444 |
| 33 | Pere Marquette | State-supported | Chicago – Grand Rapids | 1 | 176 |
| 34 | Piedmont | State-supported | Raleigh – Charlotte | 4 | 173 |
| 35 | San Joaquins | State-supported | Oakland/Sacramento – Bakersfield | 6 | 318 (Bakersfield–Oakland)280 (Bakersfield–Sacramento) |
| 36 | Silver Meteor | Long distance | New York – Miami | 1 | 1,389 |
| 37 | Silver Star | Long distance | New York – Miami | 1 | 1,522 |
| 38 | Southwest Chief | Long distance | Chicago – Los Angeles | 1 | 2,256 |
| 39 | Sunset Limited | Long distance | New Orleans – Los Angeles | 3 weekly round trips | 1,995 |
| 40 | Texas Eagle | Long distance | Chicago – San Antonio(through cars to Los Angeles on the Sunset Limited) | 1 | 1,306 (Chicago – San Antonio)2,728 (Chicago – Los Angeles) |
| 41 | Valley Flyer | State-supported | Greenfield – Springfield – New Haven | 2 | 98 |
| 42 | Vermonter | NEC; State-supported | St. Albans – Washington | 1 | 611 |
| 43 | Wolverine | State-supported | Chicago – Pontiac | 3 | 304 |